Journal: Frontiers in Oncology

Article Title: Screening and identification of novel protein markers of early-stage lung cancer and construction and application of screening models

doi: 10.3389/fonc.2025.1567673

Figure Lengend Snippet: Survival analysis of the candidate protein markers. (A) The survival curve of CLEC3B. (B) The survival curve of AOC3. (C) The survival curve of HBB. (D) The survival curve of CAT. (E) The survival curve of SEPP1. (F) The survival curve of FGA. (G) The survival curve of ORM1. HR, hazard ratio; CLEC3B, C-type lectin domain family 3 member B; AOC3, membrane primary amine oxidase; HBB, hemoglobin subunit beta; CAT, catalase; SEPP1, selenoprotein P; FGA, Fibrinogen alpha chain; ORM1, Alpha-1-acid glycoprotein 1.

Article Snippet: The levels of AOC3, CAT, CLEC3B, SEPP1, and HBB in the plasma and alveolar lavage fluid of mice were measured using ELISA (Cusabio Biotechnology, Wuhan, China).

Techniques: Membrane

Journal: Frontiers in Oncology

Article Title: Screening and identification of novel protein markers of early-stage lung cancer and construction and application of screening models

doi: 10.3389/fonc.2025.1567673

Figure Lengend Snippet: Expression of candidate protein markers in cells of different passages. Expression and comparison of AOC3, CAT, CLEC3B, and SEPP1 among saline group, DMSO group and CTPE exposure group (A) in passage 10 (* CTPE vs . Saline group, P < 0.05). (B) in passage 20 (* CTPE vs . Saline group, P < 0.05). (C) in passage 30 (* CTPE vs . Saline group, P < 0.05). (D) in passage 40 (* CTPE vs . Saline group, P < 0.05). (E) Expression and comparison of AOC3, CAT, CLEC3B, and SEPP1 between BEAS-2B and A549 (*: A549 vs . BEAS-2B, P < 0.05).

Article Snippet: The levels of AOC3, CAT, CLEC3B, SEPP1, and HBB in the plasma and alveolar lavage fluid of mice were measured using ELISA (Cusabio Biotechnology, Wuhan, China).

Techniques: Expressing, Comparison, Saline

Journal: Frontiers in Oncology

Article Title: Screening and identification of novel protein markers of early-stage lung cancer and construction and application of screening models

doi: 10.3389/fonc.2025.1567673

Figure Lengend Snippet: Expression of candidate protein markers in lung tissue of mice in different months. Expressions and comparisons of AOC3, CAT, CLEC3B, SEPP1, and HBB among normal control (NC) group, vehicle control (VC) group and CTPE exposure group (A) in the 3rd month. (B) in the 6th month. (C) in the 9th month. (D) in the 12th month (* CTPE vs . NC, P < 0.05).

Article Snippet: The levels of AOC3, CAT, CLEC3B, SEPP1, and HBB in the plasma and alveolar lavage fluid of mice were measured using ELISA (Cusabio Biotechnology, Wuhan, China).

Techniques: Expressing, Control

Journal: Communications Biology

Article Title: Crosstalk of cell death pathways unveils an autophagy-related gene AOC3 as a critical prognostic marker in colorectal cancer

doi: 10.1038/s42003-024-05980-6

Figure Lengend Snippet: a Univariate Cox analysis of CDN4 feature genes with p < 0.05 and HR > 1 in TCGA and GEO prognosis cohorts. b Mean C-index of the candidate genes. c – f Kaplan–Meier curves of overall survival with the log-rank test between the high AOC3 group and low AOC3 group in TCGA and three validation datasets. g Representative immunohistochemistry (IHC) staining images of CRC tissue microarrays in low AOC3 tumor expression and high AOC3 tumor expression tissues. h Kaplan–Meier curves of overall survival with the log-rank test between the high AOC3 H-scores group and low AOC3 H-scores group in IHC arrays. i The molecules significantly associated with AOC3 were enriched in regulation of autophagy pathway. j Correlation analysis of AOC3 with regulation of autophagy pathways score. k Differential expression of autophagy genes in different AOC3 expressions. ns fdr>0.05, *fdr < 0.05, **fdr < 0.01, ***fdr < 0.001, ****fdr < 0.0001.

Article Snippet: Immunohistochemistry (IHC) was performed using AOC3 (1:600; Cat no. 66834-1-Ig, Proteintech, Wuhan, China).

Techniques: Biomarker Discovery, Immunohistochemistry, Expressing, Quantitative Proteomics